Lazio manager Maurizio Sarri has resigned after a fifth defeat in six games in all competitions.
Monday’s 2-1 home defeat by Udinese left Lazio ninth in Serie A, seven points off the final European qualification place.
They were knocked out the Champions League last week by Bayern Munich.
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Sarri, 65, joined Lazio in 2021 and led them to second place in Serie A last season, their best league finish since they won the title in 1999-2000.
Assistant manager Giovanni Martusciello has been placed in interim charge.
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Lazio travel to third-bottom Frosinone on Saturday. They face Juventus in the semi-finals of the Italian Cup over two legs next month.